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    STUDIA CHEMIA - Issue no. Special Issue / 2010  
         
  Article:   HETEROLOGOUS EXPRESSION OF BACILLUS LICHENIFORMIS α-AMYLASE IN PICHIA PASTORIS.

Authors:  . 		 
       
         
  Abstract:  The Pichia pastoris expression system has the potential for a very high production level of foreign proteins. The α-amylase enzyme (1,4-α-D-glucan-glucanohydrolase) catalyze the endohydrolysis of 1,4-α-D-glucosidic linkages of starch and related poly- and oligosaccharides producing maltose and larger oligosaccharides. Bacillus licheniformis α-amylase (BLA) is a highly thermostable enzyme which is widely used in biotechnological processes. In this study, the gene encoding the α-amylase from Bacillus licheniformis was amplified by PCR and cloned into P. pastoris X33 host strain using the vector pPICZαA allowing methanol induced expression and secretion of the protein, using the methanol-controlled alcohol oxidase (AOX1) expression system promoter. The ultimate goal of our research is the development of a new technology for amylolytic enzyme production, applicable in fermentation processes of starch-containing materials, technology which can be applied in case of different industrial enzymes as well.

Keywords: Bacillus licheniformis, α-amylase, heterologous expression, Pichia pastoris, AOX1 promoter

 
         
     
         
         
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