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    STUDIA CHEMIA - Issue no. Special Issue / 2010  
         
  Article:   HETEROLOGOUS EXPRESSION OF GRANZYME H IN ESCHERICHIA COLI .

Authors:  . 		 
       
         
  Abstract:  Granzyme H belongs to the family of serine proteases, which plays an important role in fighting infections as part of the natural immune system. This enzyme is produced by natural killer cells and the T lymphocytes, inducing apoptosis in target cells. The precise role of the enzyme is not yet clarified; its crystal structure is unknown. The sequence encoding Granzyme H was cloned from human lymphocytes for heterologous expression. The assembled pET17cGraH expression vector contains the sequence encoding granzyme H under the control of a strong inducible promoter. Protein production was carried out in E. coli Bl21(DE3)plysS cultures.

Keywords: apoptosis, Granzyme H, heterologous expression, serine proteases

  
         
     
         
         
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