AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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STUDIA CHEMIA - Issue no. Special Issue / 2010 | |||||||
Article: |
HETEROLOGOUS EXPRESSION OF GRANZYME H IN ESCHERICHIA COLI . Authors: . |
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Abstract: Granzyme H belongs to the family of serine proteases, which plays an important role in fighting infections as part of the natural immune system. This enzyme is produced by natural killer cells and the T lymphocytes, inducing apoptosis in target cells. The precise role of the enzyme is not yet clarified; its crystal structure is unknown. The sequence encoding Granzyme H was cloned from human lymphocytes for heterologous expression. The assembled pET17cGraH expression vector contains the sequence encoding granzyme H under the control of a strong inducible promoter. Protein production was carried out in E. coli Bl21(DE3)plysS cultures. Keywords: apoptosis, Granzyme H, heterologous expression, serine proteases |
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