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The designed biosensor is based on a mutant form of green fluorescent protein (GFP). It is known that metal ions in close proximity to a cromophore group reduce the protein’ s fluorescence intensity by fluorescence quenching. Using site directed mutagenesis, we formed a metal binding site near the protein’s chromophore group. Thus, the mutant protein presents a new functional property, which allows its utilization in detection of metal ions.

Keywords: green fluorescent protein, metal binding, site-specific mutation, chromophore group, biosensor