AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Authors: DORINA CASONI, ILEANA MARIA SIMION, SIMONA CODRUȚA AURORA COBZAC, ALEXANDRA-GABRIELA KIRALY.
DOI: 10.24193/subbchem.2022.4.15
Published Online: 2022-12-30
Published Print: 2022-12-30
pp. 235-247
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Abstract. A new micro-HPTLC method assisted by image analysis technique was proposed for rapid evaluation of the total antioxidant potential (TAP) of redox-active drugs using 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2’-azinobis-3 ethylbenzothiazoline-6-sulfonic acid (ABTS*+) radicals. Colored compounds that can significantly influence the spectrophotometric and chromatographic measurements were revealed in case of selected drugs after their reaction with DPPH• and ABTS*+ radical respectively. The best chromatographic conditions were selected for accurate quantification of DPPH• and ABTS*+ radicals after their separation from the interfering compounds. The validation of the proposed method was performed in terms of linearity, limit of detection, limit of quantification, precision, and accuracy. Green and red color channel used for image processing were found to allow accurate quantification of DPPH•. For accurate quantification of ABTS*+ the red and gray channels were selected. A linear dependence of spot area/radical concentration was obtained in the range 0.78-2.76 µg⋅spot-1 and 0.21-2.49 µg/spot for DPPH• and ABTS*+ radicals respectively. The developed method was found to be accurate and precise according to values higher than 92% for recovery and less than 3% for relative standard deviation parameters (RSD%). A statistical significant correlation was obtained comparing the spectrophotometric results with the chromatographic ones obtained for selected adrenergic drugs.
Keywords: micro-HPTLC method; image analysis; total antioxidant potential; adrenergic drugs; DPPH• and ABTS*+ protocols
