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Phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) catalyzes the non-oxidative conversion of L-phenylalanine into (E)-cinnamate. PAL can be applied in organic synthesis, and can be considered also for enzyme supplementation cure for genetic disorder phenylketonuria. The aim of this study was to find optimal expression parameters of several previously cloned PAL’s (bacterial, plant and a chimera) in pBAD vectors for further functional characterization. Investigation of the expression level of PAL''''s in E. coli hosts with SDS PAGE analysis as well as activity assay of the recombinant PAL enzymes was performed.