AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Authors: NELI-KINGA OLAH, DANIELA HANGANU, EDE BODOKI, RADU OPREAN, CLAUDIA TOMA, CLAUDIU MORGOVAN, ELISABETA CHIȘE, ANDREEA BRAȘOVAN, SIMONA CODRUȚA AURORA COBZAC, SIMION GOCAN.
DOI: https://doi.org/10.24193/subbchem.2017.3.01
Published Online: 2017-09-30
Published Print: 2017-09-30
VIEW PDF: CHARACTERIZATION OF ORTHOSIPHON STAMINEUS BENTH EXTRACTS…
TLC is a powerful method used for separation of complex mixtures such as plant extracts. Employing different TLC techniques the separations can be improved. This paper presents a study of the Orthosiphon stamineus Benth extracts using isocratic reversed-phase thin layer chromatography (RP-TLC) and reversed-phase automated multiple development technique (RP-AMD). Methanol (SI) and a mixture of methanol–water–methyl acetate (SII) were used as extraction agents. Orthosiphon stamineus Benth. belongs to the Lamiaceae family. Its leaves contain rosmarinic acid, sinesetine and eupatorine as main compounds. After TLC separation the bioactive compounds from plant extracts were identified by comparison of the Rf values and in situ UV-Vis spectra with those of the standards and quantified using the calibration method. The rosmarinic acid was better extracted in the solvent mixture methanol–water–methyl acetate (10:10:80, v/v), while the sinesetine and eupatorine, which are more lipophilic, were better extracted in methanol. The study revealed the AMD technique superiority in comparison with the isocratic one.
Keywords: RP-TLC; RP-AMD; Orthosiphon stamineus Benth. extracts; rosmarinic acid; sinesetine; eupatorine.
