The STUDIA UNIVERSITATIS BABEŞ-BOLYAI issue article summary

The summary of the selected article appears at the bottom of the page. In order to get back to the contents of the issue this article belongs to you have to access the link from the title. In order to see all the articles of the archive which have as author/co-author one of the authors mentioned below, you have to access the link from the author's name.

A new simple, sensitive and selective liquid chromatography coupled with mass spectrometry (LC-MS) method for quantification of ciprofloxacin in human plasma was validated. Ciprofloxacin and ofloxacin, as internal standard, were analysed on a SB-C18 column (Agilent Technologies, 100 mm x 3 mm I.D., 3.5 μm) under isocratic conditions using a mobile phase of a 70:30 (v/v) mixture of 0.1% (v/v) formic acid in water and acetonitrile. The flow rate was 0.5 mL/min at the column temperature of 25 ºC. The detection of the analyte was in SIM mode using a triple quadrupole mass spectrometer with electrospray positive ionisation. The monitored ions were m/z 332 for ciprofloxacin and m/z for 362 for ofloxacin. The sample preparation was very simple and consisted of protein precipitation from 0.2 mL plasma using 0.4 mL of 0.05% acetic acid solution in methanol containing 0.5 μg/mL internal standard. Linear calibration curves were generated over the range of 25-5000 ng/mL with values for coefficient of determination greater than 0.999 and by using a weighted (1/c) linear regression. The lowest limit of quantification was 10 ng/ml. The values of precision (RSD%) and accuracy (relative error%) were less than 8.7% and 11.9%, for within- and between-run, respectively. The recovery of the analyte ranged between 82.5 and 91.1%. Ciprofloxacin demonstrated good stability in various conditions. The validated LC-MS method allows ciprofloxacin monitoring in human plasma during clinical treatment or other pharmacokinetics investigation.

Keywords: ciprofloxacin, LC-MS, human plasma, pharmacokinetics, therapeutic drug monitoring