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    STUDIA CHEMIA - Issue no. 3 / 2010  
         
  Article:   EXTRACTION AND CHROMATOGRAPHIC DETERMINATION OF ESSENTIALS OILS FROM OCIMUM BASILICUM L. LEAVES.

Authors:  MARIA-LOREDANA SORAN, SIMONA CODRUTA COBZAC, CODRUTA VARODI, ILDIKO LUNG, EMANOIL SURDUCAN, VASILE SURDUCAN.
 
       
         
  Abstract:  Three different techniques (maceration, sonication and microwave assisted extraction) were used for extraction of essential oils from Ocimum basilicum L. The extracts were analyzed by TLC/HPTLC technique and the fingerprint information were obtained. The GC-FID was used to characterized the extraction efficiency and for identify the terpenic bioactive compounds. The most efficient extraction technique was maceration followed by microwave and ultrasound. The best extraction solvent system was ethyl ether - ethanol (1:1, v/v). The main compounds identified in Ocimum basilicum L. extracts were: α and β-pinene (mixture), limonene, citronellol, and geraniol.

Keywords: essential oils, Ocimum basilicum L., extraction, chromatography
 
         
     
         
         
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