AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
The STUDIA UNIVERSITATIS BABEŞ-BOLYAI issue article summary
The summary of the selected article appears at the bottom of the page. In order to get back to the contents of the issue this article belongs to you have to access the link from the title. In order to see all the articles of the archive which have as author/co-author one of the authors mentioned below, you have to access the link from the author's name.
Authors: JUDITH-HAJNAL BARTHA-VARI, RENÁTA ELEKES-DARABONT, LAURA-EDIT BARABÁS, RÉKA BARABÁS.
DOI: 10.24193/subbchem.2021.1.13
Published Online: 2021-03-20
Published Print: 2021-03-30
pp. 165-178
VIEW PDF
FULL PDF
ABSTRACT: A new and efficient immobilization method of phenylalanine ammonia-lyase was obtained using hydroxyapatite (HAP) and hydroxyapatite - carbon nanotube, gelatin and chitosan - composites (HAP-CNT, HAP-GEL and HAP-CS) as support material. HAP and HAP composites were characterized by transmission electron microscopy (TEM), laser diffraction particle size analyzer and XRD measurements. The obtained nanobioconjugates were tested in the ammonia elimination reactions from L-phenylalanine (L-1). The reusability and the time-course profile of the immobilized enzyme preparation was also tested. While the biocatalyst obtained by using HAP-CS-15 as support material provided the highest conversion rate in the ammonia elimination reaction from L-1 (98,1% compared to 98,5% obtained using the non-immobilized enzyme), the most stable biocatalyst proved to be HAP-PAL, which maintained more than 80% of its initial activity even after 5 cycles of reuse.
Key words: hydroxyapatite, composites, carbon nanotubes, gelatin, chitosan, phenylalanine ammonia-lyase, immobilization, ammonia elimination.
