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    STUDIA CHEMIA - Issue no. 1 / 2014  

Authors:  .
  Abstract:   Two histidine - substituted mutants of Enhanced Green Fluorescent Protein (S202H-EGFP and S202H/Q204H-EGFP) was realized and the effect of mutation on the spectral properties of mutant proteins was examined. The denaturation of the wild type and mutant proteins in guanidine hydrochloride solution was also studied. The structural stability analysis revealed that the global structure of the protein is partially destabilized due to the performed mutations. Meanwhile the sensitivity of mutant proteins to the solvent acidity increased. It was found that with increase of proton concentration in the solvent the absorbance and the fluorescence intensity of proteins decreased.

Keywords: green fluorescent protein, histidine, fluorescence, absorbance, chromophore
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