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The chromatographic behavior of a series of amino acids compounds was investigated on silica gel chromatographic plates impregnated with various oils (paraffin, olive, sunflower and corn) and different animal fats (pig, pullet, sheep and bear) using mixture of methanol-phosphate buffer in different proportions as mobile phase. The relevance of the obtained results was evaluated by a critical comparison of the lipophilicity parameters with different theoretical lipophilicity and solubility indices. Also some correlation matrices and diagrams were developed for a comparative evaluation of the studied impregnated stationary phases. The results indicated that the oils and some animal fats (pullet and bear) impregnated silica gel plates can be a good alternative in the field of chromatographic lipophilicity estimation of amino acids. In addition, the PCA methodology proved again to offer a realistic characterization of the impregnated plates, both from the retention mechanism and lipophilicity point of view.

Keyords: TLC, lipophilicity, amino acids, impregnated TLC plates