The STUDIA UNIVERSITATIS BABEŞ-BOLYAI issue article summary

The summary of the selected article appears at the bottom of the page. In order to get back to the contents of the issue this article belongs to you have to access the link from the title. In order to see all the articles of the archive which have as author/co-author one of the authors mentioned below, you have to access the link from the author's name.

 
       
         
    STUDIA CHEMIA - Issue no. 1 / 2010  
         
  Article:   QUANTIFICATION OF PHENOBARBITAL IN HUMAN PLASMA BY LC/MS/MS FOR THERAPEUTIC DRUG MONITORING.

Authors:  LAURIAN VLASE, IOANA FELECAN, DANA MUNTEAN, DANIELA IACOB.
 
       
         
  Abstract:  A simple and sensitive LC/MS/MS method for quantification of phenobarbital and in human plasma has been developed and validated. Phenobarbital was separated under isocratic conditions using a mobile phase of 70:30 (v/v) 0.1% acetic acid and acetonitrile. In these chromatographic conditions, the retention time of phenobarbital was 1.2 min and the overall time of one analysis was 1.7 min. Plasma sample preparation consisted in protein precipitation with methanol. The detection of phenobarbital was realized in MRM mode using an ion trap mass spectrometer with electrospray negative ionization. The linearity domain was established between 2.00 and 80.00 µg/mL. Accuracy (bias%) and precision (CV%) were less than 9.8% for intra-day assay and 12.6% for inter-day assay. The recovery ranged between 95.9 and 136.4%. The method is very simple and fast and was used for therapeutic drug monitoring of phenobarbital.

Keywords: phenobarbital, LC/MS/MS, therapeutic drug monitoring
 
         
     
         
         
      Back to previous page