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    STUDIA CHEMIA - Issue no. 1 / 2005  
         
  Article:   BIOSENSOR FOR AMPEROMETRIC DETECTION OF CATECHOL BASED ON MUSHROOM TISSUE AND A METHYLENE GREEN-NAFION MODIFIED ELECTRODE.

Authors:  SONIA A. SPINEAN, GRAZIELLA LIANA TURDEAN, IONEL CĂTĂLIN POPESCU.
 
       
         
  Abstract:  An enzyme based biosensor for the amperometric detection of catechol is described. The bioelectrode architecture consists of a Nafion film, as immobilization matrix, phenothiazine dye (Methylene Green, MG) as mediator and mushroom tissue as tyrosinase source. The electrochemical and electrocatalytical behavior of the bioelectrode was investigated by cyclic voltammetry and amperometry. The biosensor has a sensitivity of 2 mA*M-1, with a detection limit of 20 M catechol and reaches 95 % of the steady-state current within 50-100 s, when the applied potential was -0.05 V vs. Ag/AgCl, KClsat.  
         
     
         
         
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