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    STUDIA BIOLOGIA - Issue no. 2 / 2007  
         
  Article:   MICROORGANISM ASSOCIATIONS WITHIN A THIN ACID SOLUTION FILM FROM AN OLD MINE IN BANAT MOUNTAINS.

Authors:  VASILE-DANIEL GHERMAN , JEAN-GABRIEL BRÉHERET, MIHAIL DRĂGAN-BULARDA. 		 
       
         
  Abstract:  The rain water slowly crosses the rock layers down to the cavity through micro cracks and it arrives on the cavity wall as a concentrated solution with a high acidity level (pH 1-1.5). This concentrated acid solution was subjected to some microbiological determinations and the identified strains were numerical estimated. These determinations were done in 2004 and 2005, during two hydrological different seasons, rainy and dry. A percentage estimate of the physiological groups has also been done. The percolating acid solution is crowded by acidophilic microorganisms with a dominating population of Acidithiobacillus ferrooxidans and six strains of Penicillium. The chemolitotrophic bacteria A. ferrooxidans provides the primary organic substance production of the community by oxidation of pyrite from the cavity walls. The six strains of Penicillium represent the heterotrophic organisms that use the organic substance from the primary producer. Between the A. ferrooxidans population and the Penicillium strain populations a close trophic relationship is established and the microorganisms number depends on the acid solution concentration.  
         
     
         
         
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