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    STUDIA BIOLOGIA - Issue no. 2 / 2007  
         
  Article:   BACTERIAL AND ENZYMATIC POTENTIAL OF THE EXPERIMENTAL PLOTS INSTALLED ON THE IRON MINE SPOILS IN IARA (CLUJ COUNTY).

Authors:  VASILE MUNTEAN, GHEORGHE GROZA.
 
       
         
  Abstract:  The paper presents the evolution of the bacterial and enzymatic potential in the soils of the experimental plots installed on the iron mine spoils in Iara, in May 2005, in the frame of a bioremediation experiment. 26 experimental plots were submitted to different treatments, and sowed with herbaceous species belonging to the families Poaceae (Festuca rubra, Festuca arundinacea, Dactylis glomerata, Lolium perenne) and Fabaceae (Onobrychis viciifolia, Trifolium repens, Trifolium pratense, Lotus corniculatus and Medicago sativa). Microbiological and enzymological analyses were carried out after three months since the experiment had been initiated, and after one year of vegetation. The microbial potential was appreciate according to the bacterial indicators of soil quality (BISQ), calculated taking into account the number of bacteria which belong to the following 5 ecophysiological groups: aerobic mesophilic heterotrophs, ammonifiers, denitrifiers, iron-reducers and sulphate reducing bacteria. The enzymatic potential was appreciate by the enzymatic indicators of soil quality (EISQ), calculated on the base of the phosphatase, catalase, actual and potential dehydrogenase activities. The most efficient treatment applied to the experimental plots proved to be the coverage with a 10-cm layer of soil: the plots covered with soil had higher values of both bacterial and enzymatic indicators of soil quality than the uncovered ones. A remarkable increase of both bacterial, and enzymatic potential has been registered in all the experimental plots in the second year of the experiment. According to the values of both the bacterial, and enzymatic indicators of soil quality after one year of vegetation, one can be stated that the most suited species to be used for bioremediation of such spoils is Medicago sativa.  
         
     
         
         
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