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    STUDIA BIOLOGIA - Issue no. 1 / 2019  
         
  Article:   CHARACTERIZATION OF HALOPHILIC CELLULASE-PRODUCING NOVEL HALOARCHAEA.

Authors:  TSUBASA ITO, HIROAKI MINEGISHI, YASUHIRO SHIMANE. 		 
       
         
  Abstract:   Cellulose is the largest biological resource on the earth. Cellulose-degrading enzymes are used in many industrial fields such as medical digestive enzymes, bioethanol, and cellulose treatment of wastewater. It is known that halophilic enzymes can be catalytically active in organic solvents and high temperature environments. Haloarchaea are adapted to a life in multiple extreme conditions, and some of them are capable of growth on cellulosic waste as carbon and energy source by producing cellulase. However, halophilic cellulases produced by haloarchaea are rarely reported. Therefore, in this study, we aimed to search for novel haloarchaeal cellulase capable of maintaining enzyme activity even under high salt concentration, high temperature and in organic solvent environments. Sixty-four cellulase-producing strains were isolated from 668 kinds of commercial salt samples. The 16S rRNA gene sequences analysis of strains 434Sel4, 434Sel6 and 434Sel7 were classified as a new genus of haloarchaea. Optimal cellulase activities from these haloarchaeal isolates were found at 60-70°C, pH 7.0-8.0 and 30% NaCl. Furthermore, high activity and stability over broad ranges of temperature (40–70°C), pH (6.0–8.0) and NaCl concentration (15–30%) were observed, showing thermostable and halostable properties of the haloarchaeal cellulases.

Keywords: cellulase, enzymes, haloarchaea 		 
         
     
         
         
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