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Authors: MIHAIL DRĂGAN-BULARDA, RAHELA CARPA, MIHAELA MIŞCA, VICENŢIU CĂLEAN.
This paper presents the results of obtaining a Bacillus subtilis strain, by UV irradiation, a strain that should produce alpha-amylase. We had in view the increase of biomass on a mineral medium with yeast extract, to which soluble starch 2% was added, and we used therefore, an installation with fermentation pot. The production of alpha-amylase was monitored, determining the maximum specific increase rate, respectively the relation between the increase of the cell mass and the enzyme production. We had also in view to obtain a raw enzymatic preparation and a partial purified one, for which Km and Vmax of reaction were determined. The enzyme production proved to be closely correlated with the quantity of biomass. The bacteria culture reached constant production efficiency after the lag phase. The raw enzymatic preparation presented a slightly higher alpha-amylase activity than the partial purified one, probably because of the starch traces present in the raw enzymatic extract.
Keywords: α-amylase, Bacillus subtilis, characterization, enzyme kinetics, fermentation, purification
