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The alga was grown using Bold nutritive solution (BBM) with continuous air stirring and illumination with 485 μmol.m^-2.s^-1, at 22⁰ C, during exponential growth. F₀ has decreased in state 2 under preillumination and low temperature, and in state 1, in the presence of DCMU and methylviologen, F₀ has been the same with the control value. F_m decreased in both state 1 and 2 which lead to a decrease of the variable fluorescence. The F_v/F_m and the quantum yield decreased significantly in both redox states, highlighting the photoinhibition of the closed reaction centers. The excitation pressure decreased, and in the presence of DBIMB, it increased in state 1, which states the growth of the closed Q_A proportion. q_P has increased in state 1 which emphasizes the growth of the opened Q_A proportion. The increase in the non-photochemical dissipation of the excitation energy is correlated with the excitation pressure. The inducement of chlorophyll fluorescence with quenching analysis on a short period of time in state 1 and 2 on whole cells marks out the proportionality ratio with the light harvesting antenna size.

Keywords: chlorophyll fluorescence; photochemical activity; photosynthetic inhibitors; state 1- state 2 transitions.