AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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STUDIA CHEMIA - Ediţia nr.3 din 2011 | |||||||
Articol: |
ADSORBŢIA METHEMOGLOBINEI DE CABALINĂ PE STICLA BIOACTIVĂ LA CONCENTRAŢIE MARE DE SARE, STUDIATE DE EPR ŞI SPECTROSCOPIE FTIR . Autori: CRISTINA GRUIAN, HEINZ-JÜRGEN STEINHOFF, SIMION SIMON. |
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Rezumat: Although protein adsorption has been intensively studied in the last years, conformational changes which are likely to appear on the structure of the protein upon adsorption are difficult to evidence directly. Combined SDSL and EPR spectroscopy were applied to study adsorption of horse mehemoglobin methemoglobin on bioactive glass in solution with high salt concentration (500mM NaCl). Further studies using FTIR spectroscopy aimed at identifying changes in the secondary structure of the protein upon the adsorption process. EPR spectra of methemoglobin spin labeled at position β-93 in solution were compared to those obtained after adsorption to extract structural information. A consistent analysis of EPR spectra revealed that the adsorption of methemoglobin is influenced by the chemical treatment applied to the surface of bioactive glass. From the FTIR spectra details concerning the changes of secondary structure of the protein after 2 hours of sample immersion in protein solution were obtained. Keywords: site-directed spin labeling, FTIR spectroscopy, protein adsorption, bioactive glasses |
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