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    STUDIA PHYSICA - Issue no. 1 / 2009  
         
  Article:   SEA BUCKTHORN OIL STUDY BY GC/MS AND IR.

Authors:  CORNELIA MESAROS, ANDREEA IORDACHE, MONICA CULEA, CORA CRĂCIUN, ONUC COZAR, RADU FECHETE, EUGEN CULEA. 		 
       
         
  Abstract:  A sensitive analytical method, very useful for determination of fatty acids, vitamin F and E and sterols, in biological samples, was developed by gas chromatography/ mass spectrometry (GC/MS). The method was applied for testing fatty acids, vitamin E, sterols, after the treatment of sea buckthorn oil (Hippophäe rhamnoides L) at different kGy doses with accelerated electrons. The internal standard used for fatty acid methyl esters determination was C11:1. Liposoluble compounds from sea buckthorn oil represent a polivitaminic complex with regenerator action upon the cellular metabolism. Our method is suitable for fatty acid screening and quantitative determination and to state the changes of the nutrients after treatment with accelerated electron. GC/MS analyses were performed on a Trace DSQ. Comparisons with other methods, as HPLC and by IR for sea buckthorn oil changes are presented.

Keywords: GC/MS analyses , sea buckthorn oil, fatty acid 		 
         
     
         
         
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