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    STUDIA CHEMIA - Issue no. 4 / 2015  
         
  Article:   DEVELOPMENT OF AN UPLC METHOD FOR SIMULTANEOUS DETERMINATION OF TARTRAZINE, CONGO RED AND METHYL ORANGE .

Authors:  . 		 
       
         
  Abstract:   An UPLC method was developed to determine the concentrations of tartrazine (TAR), Congo red (CR), and methyl orange (MO) in aqueous mixtures. The chromatographic method was optimized considering the temperature of the column, the composition of mobile phases and the gradient program. The chosen method has allowed the separation and identification of TAR, CR, and MO from aqueous samples in 2 min. The calibration plots (R2> 0.991) were linear over the ranges 0.5 – 50 µg/mL. Accuracy of the method was investigated, by applying recovery tests, with average recovery higher than 99%. The precision analysis included an intra-day variation, for which RSD (%) had values lower than 0.94, and an inter-day variation with RSD (%) values lower than 1.07. The developed method was successfully tested on water samples collected from a river nearby a textile industry plant.

Keywords: azo dyes; Congo red; methyl orange; tartrazine; UPLC
 
         
     
         
         
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