AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Authors: DORINA CASONI, MIHAELA BADEA, ILDIKO BROS, SIMONA CODRUTA AURORA COBZAC.
DOI: https://doi.org/10.24193/subbchem.2017.3.07
Published Online: 2017-09-30
Published Print: 2017-09-30
VIEW PDF: INVESTIGATION ON IMAGE PROCESSING PARAMETERS…
Based on advantages of available modern image processing techniques, thin-layer chromatography combined with image analysis becomes a promising alternative for the mycotoxins determinations in various foodstuffs. For this, the effect of digitizing channel selection and image processing parameters on aflatoxin B2 (AB2) and ochratoxin A (OTA) determination was evaluated and the obtained results were compared with those of classical photodensitometric analysis. The best exciting wavelength for OTA was found to be λexcitation=333nm while for AB2 λexcitation=365nm. Low detection and quantification limits were determined (LODOTA=0.310ng/spot; LOQOTA=0.616ng/spot, LODAB2=0.557ng/spot, LOQAB2=1.098ng/spot). For image processing, the parameter with the highest influence upon the regression calibration curve was brightness and the best digitizing channel was found to be the green one. First-order calibration curve with a good determination coefficient (R2>0.99) were obtained on the working range of 2.5-50ng/spot. Although image processing techniques do not provide higher LOD and LOQ values than classical photodensitometry, this method offers the advantage of simultaneous quantification of both mycotoxins.
Keywords: aflatoxin B1, ochratoxin A, TLC; densitometry, image processing
