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    STUDIA CHEMIA - Issue no. 3 / 2012  
         
  Article:   POTENTIAL BACTERIAL SOIL INOCULANT MADE UP BY RHODOCOCCUS SP. AND PSEUDOMONAS SP. FOR REMEDIATION IN SITU OF HYDROCARBON – AND HEAVY METAL POLLUTED SOILS.

Authors:  SZABOLCS LÁNYI.
 
       
         
  Abstract:  

Since bioremediation is an environmentally friendly, cost effective approach to treat hydrocarbon-contaminated soils, in this study our aim was to develop a bacterial soil inoculant applicable in elimination of aliphatic, simple- and polyaromatic hydrocarbons from soils. To reach our goal two bacterial strains, Pseudomonas fluorescens BBN1 and Rhodococcus qingshengii BBG1, were selected to investigate their hydrocarbon-degrading abilities at DNA level and in microcosm experiments followed by GC-FID, as well as their ability to tolerate heavy-metal concentrations. Our results revealed that the proposed bacterial soil inoculant is suitable for elimination of aliphatic, monoaromatic and polycyclic aromatic hydrocarbons from soil samples, reaching degradation rates of 95% (n-dodecane), 66% (toluene), 70% (naphthalene) and 67% (phenanthrene) of initial pollutant concentrations within 42 days.

Keywords: bioaugmentation, in situ bioremediation, PAHs, catA, C23O, alkB, GC-FID

 
         
     
         
         
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