AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Authors: ȘTEFANA STĂCESCU, GABRIEL HANCU, LASZLO GAGYI, RUXANDRA MARIA SOARE, HAJNAL KELEMEN.
DOI: https://doi.org/10.24193/subbchem.2017.2.14
Published Online: 2017-06-20
Published Print: 2017-06-30
VIEW PDF: SIMULTANEOUS DETERMINATION OF HYDROCHLOROTHIAZIDE AND TELMISARTAN…
Fixed-dose combinations of an angiotensin II receptor antagonist (telmisartan) and a thiazide diuretic (hydrochlorothiazide) provide an effective antihypertensive therapy while promoting patient compliance with the convenience of once-daily single dose administration. The current study investigated two capillary electrophoretic methods: a capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography one (MEKC) for the simultaneous determination of hydrochlorothiazide and telmisartan. In order to optimize the analytical conditions, efforts were focused on establishing the influence of several experimental parameters on the separation: buffer composition, concentration and pH, applied voltage, temperature, injection pressure and time. The separation of the two analytes can be achieved by CZE using a 25 mM phosphate buffer at pH 2.50 and by MEKC using a 50 mM borate buffer at pH 9.50 containing 25 mM sodium dodecyl sulfate as surfactant. The analytical performance of the two methods was verified in terms of their linearity, precision, accuracy and robustness, and the methods were applied for the determination of the analytes from fixed-dose combinations.
Keywords: hydrochlorothiazide, telmisartan, fixed dose combinations, capillary electrophoresis, pharmaceutical preparations
