AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Authors: BIANCA STOEAN, LUIZA GAINA, EMESE GAL, CASTELIA CRISTEA, TAMAS LOVASZ, LUMINITA SILAGHI-DUMITRESCU.
DOI: 10.24193/subbchem.2021.2.05
Published Online: 2021-06-30
Published Print: 2021-06-30
pp.59-66
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ABSTRACT: The interaction of (E)-4-(2-(7-bromo-10-ethyl-10H-phenothiazin-3-yl)vinyl)-1-methylpyridin-1-ium (PVP) dyes with calf-thymus DNA was examined by spectrophotometric UV-vis absorption/emission titration and competitive ethidium bromide displacement. The association constant values were situated in 106 M-1 range, typical to cationic dyes containing the monocyanine chromophore system. The variation of the counterion of the cationic PVP dye from iodide to tetrafluoroborate produced dissimilarities in the interaction pattern with DNA. The PVP iodide competitively displaced the ethidium bromide (EB) from the EB-DNA system, thus suggesting the intercalation ability of the dye, while the PVP tetrafluoroborate did not supported the intercalation of the dye.
Key words: cyanine dyes, phenothiazine, DNA binding, association constant
