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    STUDIA CHEMIA - Issue no. 2 / 2016  
         
  Article:   DETECTION OF OCHRATOXIN A IN INSTANT COFFEE BY UPLC-MS/MS.

Authors:  . 		 
       
         
  Abstract:   A rapid, accurate and economic procedure has been applied to extract and analyze trace level of ochratoxin A (OTA) in instant coffee. The OTA was extracted and cleaned-up by methanol extraction followed by retention on IRA-400 anion exchanger and quantified by Ultra Performance Liquid Chromatography (UPLC) with MS/MS detection. Under optimum conditions, the detection limit (S/N = 3) was 0.05 ng/g and recoveries of OTA at spiking levels of 5, 10, and 20 ng/mL, were 82.67 ± 8.05%, 96.87 ± 4.63%, and 98.25 ± 4.46% respectively.

Keywords: ochratoxin A; mycotoxin; instant coffee; contamination; UPLC-MS.
 
         
     
         
         
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