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    STUDIA CHEMIA - Issue no. 2 / 2012  
         
  Article:   MULTI-SUBSTRATE KINETIC RESOLUTION SCREENING METHOD FOR LIPASE BIOCATALYSTS.

Authors:  LÁSZLÓ POPPE.
 
       
         
  Abstract:  Development of efficient screening methods has increasing significance in rapid evaluation of novel biocatalysts. Our study reveals the scopes and limitations of a novel GC-based multi-substrate screening method for initial characterization of the activity and selectivity of lipase biocatalysts. The multi-substrate kinetic resolution of four different racemic alcohols 1-4a by native and immobilized biocatalysts were analyzed by GC using enantiomer selective stationary phase.

Keywords: lipase, immobilization, biocatalysis, multi-substrate screening
 
         
     
         
         
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