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    STUDIA CHEMIA - Issue no. 1-2 / 2001  
         
  Article:   SELECTIVE SEPARATION OF AMINOACIDS BY REACTIVE EXTRACTION.

Authors:  CORNELIU ONISCU, ANCA-IRINA GALACTION, DAN CAȘCAVAL.
 
       
         
  Abstract:  The method of reactive extraction with di-(2-ethylhexyl)phosphoric acid (D2EHPA) for the separation of a range of amino acids is studied. Amino acids are extracted by means of an interfacial reaction of ionic exchange type, if the amino acid exists as a cation in aqueous solution. The separation yield is controlled by the pH value of the aqueous phase, which is due to the acidic or basic character of each amino acid. The results obtained on the individual reactive extraction indicated the possibility of the amino acids selective separation as a function of the pH value of aqueous solution and the acidic or basic character of each amino acid. Thus, an operation flow for selective separation has been elaborated and demonstrated experimentally. Using multistage extraction, the total separation of the following amino acids groups has been performed: neutral amino acids (l-glycine, l-alanine, l-tryptophan) at pH = 5 - 5.5 (nine extraction stages), basic amino acids (l-lysine, l-arginine) and l-cysteine at pH = 4 - 4.5 (ten extraction stages), l-histidine at pH = 3 - 3.5 (five extraction stages), and acidic amino acids (l-aspartic acid, l-glutamic acid) at pH = 2 - 2.5 (three extraction stages). The proposed extraction method can be developed and used for the selective separation of amino acids from fermentation broths or protein hydrolysates.  
         
     
         
         
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