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    STUDIA CHEMIA - Issue no. 1-2 / 1999  
         
  Article:   BIOSENSORS FOR PHENOL DERIVATIVES USING ELECTROCHEMICAL AND BIOCHEMICAL SIGNAL AMPLIFICATION.

Authors:  SARMIZA ELENA STANCA, IONEL CĂTĂLIN POPESCU, LIVIU ONICIU. 		 
       
         
  Abstract:  Three different approaches to enhance the sensitivity of tyrosinase (PPO) based biosensor for the amperometric detection of phenols have been compared. An electrochemical approach, involving ferrocyanide as mediator for the detection of enzymatically produced o-quinone, was tested using a monoenzyme bioelectrode for phenol. In order to investigate the biochemical approach, based on the cooperative functioning of tyrosinase and D-glucose dehydrogenase (GDH) or tyrosinase and horseradish peroxidase (HRP), two bienzyme electrodes were constructed for L-tyrosine detection at -180 mV vs. SCE. For monoenzyme bioelectrode, as well as for bienzyme bioelectrodes, the enzymes were immobized in agar-agar gel. The highest signal amplification factor (74), was observed for the PPO-GDH couple, while that recorded for PPO-HRP couple and PPO-ferrocyanide system were 32 and 4, respectively.  
         
     
         
         
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