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Microalgae, the third generation biodiesel feedstock, have emerged as one of the most promising alternative sources of lipids that can be used in the production of biodiesel due to their advantages over conventional crops. The aim of this study was to obtain Nannochloropsis oculata microalgae oil using hexane extraction methods together with fatty acid characterization for biodiesel application. The chemical composition of microalgae showed a high total lipid content, N. oculata microalgae being a potential feedstock for biodiesel production. For algal oil extraction, dynamic extraction with Soxhlet apparatus proved more efficient, with a lipid yield of 0.190 g/g dried microalgae comparatively to only 0.136 g lipid/g dried microalgae for static hexane extraction. The main fatty acids for N. oculata microalgae are palmitic acid, palmitoleic acid, eicosatrienoic acid and eicosapentaenoic acid, the latest being also the major constituent, with a value of 48.86% (w/w).

Keywords: microalgae, biodiesel, oil extraction, lipids, fatty acids