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    STUDIA BIOLOGIA - Issue no. Sp.%20issue / 2015  
         
  Article:   MIO-ENZYME TOOLBOX: CLONING, EXPRESSION AND PURIFICATION OF RECOMBINANT RTPAL.

Authors:  ALINA FILIP, LÁSZLÓ CSABA BENCZE, CSABA PAIZS, LÁSZLÓ POPPE, FLORIN DAN IRIMIE.
 
       
         
  Abstract:  VIEW PDF: MIO-ENZYME TOOLBOX: CLONING, EXPRESSION AND PURIFICATION OF RECOMBINANT RTPAL

The recent advances in the molecular engineering of aromatic ammonia lyases (ALs) and aminomutases (AMs) attracted increased interest towards their applications in the treatment of phenylketonuria and/or the synthesis of non-natural amino acids. Herein we describe the cloning, isolation and purification of recombinant L-phenylalanine ammonia-lyase from Rhodosporidium toruloides for future biocatalytic and therapeutic applications.

Keywords: MIO–enzyme, non-natural amino acid, phenylalanine ammonia-lyase, phenylketonuria
 
         
     
         
         
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