AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
The STUDIA UNIVERSITATIS BABEŞ-BOLYAI issue article summary
The summary of the selected article appears at the bottom of the page. In order to get back to the contents of the issue this article belongs to you have to access the link from the title. In order to see all the articles of the archive which have as author/co-author one of the authors mentioned below, you have to access the link from the author's name.
Authors: BOGDAN M. BOȘCA, AUGUSTIN C. MOȚ.
Published Online: 2021-06-30
Published Print: 2021-06-30
pp. 20
VIEW PDF
FULL PDF
ABSTRACT: Selenium is an essential trace element in animals and humans. It has chemical properties similar to sulfur. In plants, selenium is involved in several important biochemical routes, including antioxidative processes (Germ et al., 2007). In animals and humans, compared to other micronutrients, there is a much thinner line between the normal concentration and the toxic amount of selenium. The inorganic forms of selenium (selenate, selenite) present a much higher toxicity than its organic combinations (e. g. selenocysteine, selenomethionine) (Herrero Latorre et al., 2013). Thus, a sensitive method is required for detection of selenium species in biological samples. A distinct method is represented by the formation of piazselenol. This kind of complexes represent the product of reaction between an aromatic o-diamine and Se(IV) species, in acidic conditions. This study describes an HPLC-based optimized, sensitive and selective method for determination of selenium in biological samples. The analyzed Allium species were grown in our laboratory in a phytochamber. Before the analysis procedures, the biofortified Allium samples have been mineralized and pre-concentrated using solid-phase microextraction technique. This analytical step affords an excellent precision and accuracy as it removes the interferences form a typical biological sample, thus allowing a limit of detection as low as 0.5 ng/mL Se.
Key words: Allium, biofortification, piazselenol, selenium determination
