AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Authors: OANA CIUZAN, DORU PAMFIL.
Arabidopsis thaliana, a small spring annual plant belonging to the Brassicaceae family, is used since the 1940s as a model in plant genetics and biotechnology due to some advantages, like small genome, large production of seeds and short life cycle. From the three major ecotypes found in the spontaneous flora (Columbia, Landsberg and Wassilewskija) a series of loss of function and gain of function mutants were created since the 1960s in order to answer questions related to several genes and proteins involved in the physiological mechanisms of plants. The most attention was laid on the genes and proteins involved in plant adaptation to abiotic and biotic stress conditions. The glycine-rich RNA-binding proteins are a major class of proteins found in superior plants that are known to have a big implication in plant adaptation to low temperatures and other osmotic and oxidative stresses. The main focus of this research was to assess the involvement of two glycine-rich RNA-binding proteins (GRP2 and GRP7) in seed germination capacity under salt stress conditions. The plant material was composed out of seed batches obtained from the T-DNA insertion Arabidopsis thaliana mutant lines labelled grp2 (knock-out mutant for the glycine-rich RNA-binding protein 2), grp7-1 (knock-out mutant for the glycine-rich RNA-binding protein 7), WS7ox (overexpressor for the GRP7 protein) and the Col0 ecotype, which was used as control. For the germination assay, three different concentrations (100 mM, 150 mM and 200 mM) of NaCl were used, with whom the control medium was supplemented. The control medium chosen consisted of ½ MS (Murashige & Skoog) vitamin medium without sugar. Fifty seeds were placed on each petri dish and they were assessed for seven days. After this period, the germination rate was estimated. Three independent replicates were used and the statistical analysis was obtained with the help of the trial version from GraphPad Prism software. The results obtained showed that the seeds belonging to the grp2 mutant were not affected by the high salt concentration, in contrast to the ones obtained from the grp7-1 mutant and WS7ox line. Also, in comparison to the wild-type, the germination rate of these seeds was higher. As a conclusion, we can assume that even though the two proteins taken under observation belong to the same class and family, they have different roles regarding the seed germination capacity.
