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Authors: ÉVA DOMOKOS-SZABOLCSY, R. PINTÉR, P. ESZENYI, ATTILA SZTRIK, J. PROKISCH, FÁRI MIKLÓS GÁBOR.
After bananas and mangoes the pineapple is the third most economically important tropical fruits in the world. The pineapple is routinely propagated vegetatively however in vitro micropropagation of it has many advantages over conventional methods. For instance, this technique allows an efficient and rapid increase of selected elite pineapple varieties. At the same time in vitro propagation can provide opportunity for controlled biofortification. This is important whereas pineapple is excellent source of several vitamins however it contains small amount microelements especially, selenium. Based on these knowledge, the goal of our study was to see if the sodium-selenate and the red elemental selenium nanoparticles (nanoSe) as inorganic selenium forms are able to influence the rooting and growth of pineapple in vitro culture combining with plant hormones (NAA or TIBA). The experimental data clearly indicated that the nanoSe is better tolerated selenium form than the selenate. The selenate in 10 mg L-1 concentration inhibited the root developing and shoot growth, and the plantlets competely died by 100 mg L-1 of it. However, no toxic symptoms were shown in case of nanoSe in the applied concentration range (1-100 mg L-1) regardless of rooting media composition. Both of two selenium forms could uptake in pineapple in vitro culture however selenate accumulated in higher amount than nanoSe applied the same concentration in the media. Acknowledgements. This research was realized in the frames of TÁMOP 4.2.4. A/2-11-1-2012-0001 National Excellence Program – Elaborating and operating an inland student and researcher personal support system. The project was subsidized by the European Union and co-financed by the European Social Fund. Additional financial support is also gratefully acknowledged for the MOP Biotech Co Ltd. (Nyíregyháza, Hungary) and Ereky Foundation (Debrecen, Hungary).
