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Authors: LIA MLADIN, NORA PRODAN, COSMIN IONEL SICORA.
The global warming can determine the alteration of normal functionality of the ecosystems, threatening with extinction a significant number of species. Most important environmental factors inducing different types of stresses on plant photosynthesis are: temperature, excessive light, CO2 concentration. Destruction of the ozone layer that absorbing short wavelength UV radiation, increases the amount of UV-B radiation reaching the Earth’s surface. These radiations can cause damage of photosystem I and II, carbon fixation, low levels of chlorophyll and sucrose in plants. The study of photosynthesis utilizing chlorophyll fluorescence measurements is one of the most modern techniques used by plant physiologists and ecophysiologists. The modern fluorometers can measure the chlorophyll fluorescence in the laboratory and in the field. For the study of UV-B stress on Eruca sativa we have utilized the MONI PAM fluorometer that monitors the efficiency of PS II in greenhouse conditions under normal and enhanced UV-B irradiation. The monitored parameters are: PAR (Photosynthetic Active Radiation), basic fluorescence level (F) maximal fluorescence level (FM´), ETR (Electron Transport Rate), YII (efficiency of Photosystem II). Measurements were done on control samples, plants treated with low UV-B radiation and high UV-B radiation producing a wide range of adaptative responses including irreversible damages of photosynthetic processes.
