AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Authors: GABRIELA N. ŢENEA, JOERG SPANTZEL, LAN-YING LEE, HEIKO OLTMANNS, STANTON B. GELVIN.
Agrobacterium-mediated genetic transformation is the dominant technology used for many years in production of genetically modified transgenic plants. Recent study has demonstrated the implication of plant genes and proteins in Agrobacterium mediated transformation. We investigated the role of several histone proteins and an anti-silencing factor A (SGA1) in plant transformation. Transgenic Arabidopsis plants containing additional copies of cDNAs encoding histone H2A (HTA), histone H4 (HFO), or SGA1 displayed increased susceptibility to transformation. Over-expression of all tested histone H2B (HTB) and most histone H3 (HTR) cDNAs did not increase transformation. A parallel increase in transient gene expression was observed when the histone HTA or HFO cDNAs were co-transfected, together with a plant active gusA gene, into tobacco protoplasts. An increase in gusA transcripts when the histone HTA1 cDNA was over-expressed in protoplasts was also detected. No such increase in gusA activity was seen when a SGA1 cDNA was co-transfected with a gusA gene into BY-2 protoplasts. Over-expression of histone or SGA1 cDNAs does not increase expression of a previously integrated transgene, nor could HTA1 reverse silencing. These data suggests that histones may increase transgene expression by working directly on the promoter of incoming DNA, or that histones may play a role in stabilizing transgene DNA (and thereby transgene expression) during the initial stages of transformation and SGA1 might lead to enhanced plant transformation by allowing T-DNA and complexed proteins greater access to plant target DNA, thus facilitating T-DNA integration.
