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    STUDIA BIOLOGIA - Issue no. 1 / 2008  
         
  Article:   A MOLECULAR APPROACH TO INVESTIGATION OF CYANOBACTERIAL DIVERSITY FROM MARGHITA AND ROŞIORI THERMOMINERAL DRILLINGS (BIHOR COUNTY, ROMANIA).

Authors:  CRISTIAN COMAN, BOGDAN DRUGĂ, ADRIANA BICA, ANA NICOARĂ, NICOLAE DRAGOŞ. 		 
       
         
  Abstract:  Understanding the relationships among species in different microbial communities, in time, has proved to be quite a challenge. The fact that the majority of species are not culturable in laboratory conditions and determination based on morphological characteristics is sometimes very difficult, emphasized the need of a new approach in this type of studies. Based on a solid biomarker – the gene for 16S rRNA and 16S-23S ITS region – two specific cyanobacterial mats associated to Marghita and Roşiori thermomineral drillings were investigated using molecular techniques. The 16S rRNA-ITS fragments from the entire community genomic DNA were amplified through PCR using specific cyanobacterial primers, the fragments were cloned and a restriction analysis was performed. The restriction maps obtained revealed the presence of 13 groups 16S RNA-ITS profiles for Marghita and 2 groups 16S RNA-ITS profiles for Roşiori, suggesting the presence of a similar number of distinct operational taxonomic units (OTU’s). Distinct fragments were sequenced and partial 16S rRNA sequences obtained were compared to the ones stored in public databases (NCBI GenBank) using blastn algorithm for specie identification. At the same time a phylogenetic analysis was conducted.  
         
     
         
         
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