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    STUDIA BIOLOGIA - Issue no. 1 / 2008  
         
  Article:   THE MOLECULAR DIVERSITY OF CYANOBACTERIAL MATS ASSOCIATED WITH THERMAL SPRINGS IN NORTH-WESTERN ROMANIA.

Authors:  BOGDAN DRUGĂ, IULIA SOFRONIE, IOANA VĂSAR, CRISTIAN COMAN, ADRIANA BICA, ANA NICOARĂ, NICOLAE DRAGOŞ. 		 
       
         
  Abstract:  In this study, the diversity of the cyanobacterial communities associated with the thermo-mineral springs was analyzed. We used a molecular technique based on the generation of different electrophoretic patterns, subsequent to a DNA digestion with restriction enzymes. The target DNA fragment which was amplified through PCR was the 16S rRNA / ITS gene, a well known molecular marker used in the phylogenetic studies of the prokaryotes. The 16S rRNA / ITS gene wes cloned in E. coli cells, using a plasmidic vector. Eventually, we have obtained 24 to 45 different clones for the 4 cyanobacterial communities. The target DNA fragments were re-amplified through PCR using the plasmids as template DNA, and then the 16S rRNA / ITS gene copies were digested with several restriction enzymes. Of all, only the TaqI restriction enzyme has generated unambiguous electrophoretic bands, and specific patterns. The distances the digested DNA fragments have migrated into the electrophoretic gel were measured. The number of distinct electrophoretic patterns is considered the same with the number of different cyanobacterial species from a related mat.  
         
     
         
         
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