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    STUDIA CHEMIA - Ediţia nr.1 din 2010  
         
  Articol:   A NEW LC/MS/MS METHOD FOR DETERMINATION OF LISINOPRIL IN HUMAN PLASMA.

Autori:  LAURIAN VLASE, DANIELA-SAVETA POPA, DANA MUNTEAN, MARCELA ACHIM. 		 
       
         
  Rezumat:  A simple and sensitive liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) method for the quantification of lisinopril in human plasma was developed and validated. The separation was performed on a Zorbax SB-C18 column under isocratic conditions using a mobile phase of 11:89 (v/v) acetonitrile and 0.1% (v/v) trifluoroacetic acid in water at 45 ºC with a flow rate of 1 mL/min. A 1% ammonia solution in acetonitrile was added post column. The detection of lisinopril was performed in multiple reaction monitoring (MRM) mode using an ion trap mass spectrometer with electrospray positive ionisation. The human plasma samples (0.25 mL) were deproteinized with 12% perchloric acid in water and aliquots of 15 μL from supernatants obtained after centrifugation were directly injected into the chromatographic system. The method shows a good linearity (r > 0.9946), precision (CV < 11.3 %) and accuracy (bias < 7.0 %) over the range of 1.29-129 ng/mL plasma. The lower limit of quantification (LLOQ) was 1.29 ng/mL and the recovery was between 97.5-105.9 %. The method is not expensive, it needs a minimum time for plasma sample preparation and has a run-time of 5.0 min for instrument analysis (retention time of lisinopril was 4.5 min). The developed and validated method is very simple, rapid and efficient, with wide applications in clinical level monitoring, pharmacokinetics and bioequivalence studies of lisinopril.

Keywords: lisinopril, LC/MS/MS, therapeutic drug monitoring
  
         
     
         
         
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